Airborne measurements of western U.S. wildfire emissions: Comparison with prescribed burning and air quality implications

Wildfires emit significant amounts of pollutants that degrade air quality. Plumes from three wildfires in the western U.S. were measured from aircraft during the Studies of Emissions and Atmospheric Composition, Clouds and Climate Coupling by Regional Surveys (SEAC4RS) and the Biomass Burning Observation Project (BBOP), both in summer 2013. This study reports an extensive set of emission factors (EFs) for over 80 gases and 5 components of submicron particulate matter (PM1) from these temperate wildfires. These include rarely, or never before, measured oxygenated volatile organic compounds and multifunctional organic nitrates. The observed EFs are compared with previous measurements of temperate wildfires, boreal forest fires, and temperate prescribed fires. The wildfires emitted high amounts of PM1 (with organic aerosol (OA) dominating the mass) with an average EF that is more than 2 times the EFs for prescribed fires. The measured EFs were used to estimate the annual wildfire emissions of carbon monoxide, nitrogen oxides, total nonmethane organic compounds, and PM1 from 11 western U.S. states. The estimated gas emissions are generally comparable with the 2011 National Emissions Inventory (NEI). However, our PM1 emission estimate (1530 ± 570 Gg yr-1) is over 3 times that of the NEI PM2.5 estimate and is also higher thanthe PM2.5 emitted from all other sources in these states in the NEI. This study indicates that the source of OA from biomass burning in the western states is significantly underestimated. In addition, our results indicate that prescribed burning may be an effective method to reduce fine particle emissions

Path loss modeling for vehicular system performance and communicaitons protocols evaluation

Vehicular communications are receiving considerable attention due to the introduction of the intelligent transportation system (ITS) concept, enabling smart and intelligent driving technologies and applications. To design, evaluate and optimize ITS applications and services oriented to improve vehicular safety, but also non-safety applications based on wireless systems, the knowledge of the propagation channel is vital. In particular, the mean path loss is one of the most important parameters used in the link budget, being a measure of the channel quality and limiting the maximum allowed distance between the transmitter (Tx) and the receiver (Rx). From a narrowband vehicular-to-vehicular (V2V) channel measurement campaign carried out at 5.9 GHz in three different urban environments characterized by high traffic density, this paper analyzes the path loss in terms of the Tx-Rx separation distance and fading statistics. Based on a linear slope model, values for the path loss exponent and the standard deviation of shadowing are reported. We have evaluated the packet error rate (PER) and the maximum achievable Tx-Rx separation distance for a PER threshold level of 10% according to the digital short-range communications (DSRC) specifications. The results reported here can be incorporated in an easy way to vehicular networks (VANETs) simulators in order to develop, evaluate and validate new protocols and systems architecture configurations under realistic propagation conditions.Fernández González, HA.; Rubio Arjona, L.; Reig, J.; Rodrigo Peñarrocha, VM.; Valero-Nogueira, A. (2013). Path loss modeling for vehicular system performance and communicaitons protocols evaluation. Mobile Networks and Applications. 18(6):755-765. doi:10.1007/s11036-013-0463-xS755765186Gallager B, Akatsuka H, Suzuki H (2006) Wireless communications for vehicle safety: radio link performance and wireless connectivity. IEEE Veh Technol Mag 1(4):4–24Rubio L, Reig J, Fernández H (2011) Propagation aspects in vehicular networks, Vehicular technologies. Almeida M (ed) InTechWang C-X, Vasilakos A, Murch R, Shen SGX, Chen W, Kosch T (2011) Guest editorial. Vehicular communications and networks – part I. IEEE J Select Areas Commun 29(1):1–6ASTM E2213-03 (2003) Standard specification for telecommunications and information exchange between roadside and vehicle systems – 5 GHz band Dedicated Short Range Communications (DSRC) Medium Access Control (MAC) and Physical Layer (PHY) specifications. American Society for Testing Materials (ASTM), West ConshohockenIEEE 1609 – Family of Standards for Wireless Access in Vehicular Environments (WAVE). [Online]. Available: http://www.standards.its.dot.govETSI TR 102 492–2 Part 2 (2006) Technical characteristics for Pan European Harminized Communications Equipment Operating in the 5 GHz frequency range intended for road safety and traffic management, and for non-safety related ITS applications, European Telecommunications Standard Institute (ETSI), Technical Report, Sophia Antipolis, FranceThe Car-to-Car Communication Comsortium (C2CC): http:/www.car-to-car.orgMecklenbräuker C, Molisch A, Karedal J, Tufvesson F, Paier A, Bernado L, Zemen T, Klemp O, Czink N (2011) Vehicular channel characterization and its implications for wireless system design and performance. IEEE Proc 99(7):1189–1212Ghafoor KZ, Bakar KA, Lloret J, Khokhar RH, Lee KC (2013) Intelligent beaconless geographical routing for urban vehicular environments. Int J Wireless Netw 19(3):345–362Ghafoor KZ, Lloret J, Bakar KA, Sadiq AS, Mussa SAB (2013) Beaconing approaches in vehicular ad hoc networks: a survey. Int J Wirel Pers Commun. Published Online (May 2013)Michelson DG, Ghassemzadeh SS (2009) New directions in wireless communications, Springer Science+Busines Media (Chapter 1)IEEE 802.11p (2010) Wireless LAN Medium Access Control (MAC) and Physical Layer (PHY) Specifications Amendment 6: Wireless Access in Vehicular Environments, Institute of Electrical and Electronic Engineers (IEEE), New York, USA.Karedal J, Czink N, Paier A, Tufvesson F, Moisch AF (2011) Path loss modeling for vehicle-to-vehicle communications. IEEE Trans Veh Technol 60(1):323–327Cheng L, Henty B, Stancil D, Bai F, Mudalige P (2007) Mobile vehicle-to-vehicle narrow-band channel measurement and characterization of the 5.9 GHz dedicated short range communication (DSRC) frequency band. IEEE J Select Areas Commun 25(8):1501–1516Cheng L, Henty B, Cooper R, Stancil D, Bai F (2008) Multi-path propagation measurements for vehicular networks at 5.9 GHz. IEEE Wireless Communications and Networking Conference, pp. 1239–1244Tan I, Tang W, Laberteaux K, Bahai N (2008) Measurement and analysis of wireless channel impairments in dsrc vehicular communications. IEEE International Conference on Communications, pp. 4882–4888.Campuzano AJ, Fernández H, Balaguer D, Vila-Jiménez A, Bernardo-Clemente B, Rodrigo-Peñarrocha VM, Reig J, Valero-Nogueira A, Rubio L (2012) Vehicular-to-vehicular channel characterization and measurement results. WAVES 4(1):14–24Kunisch J, Pamp J (2008) Wideband car-to-car radio channel measurements and model at 5.9 GHz. IEEE 68th Vehicular Technology Conference, pp. 1–5Gozalvez J, Sepulcre M (2007) Opportunistic technique for efficient wireless vehicular communications. IEEE Veh Technol Mag 2(4):33–39Zang Y, Stibor L, Orfanos G, Guo S, Reumerman H (2005) An error model for inter-vehicle communications in highway scenarios at 5.9 GHz. Proc. Int. Workshop on performance evaluation of wireless ad hoc, sensor, and ubiquitous networks, pp. 49–5

Cardiosphere-derived cells suppress allogeneic lymphocytes by production of PGE2 acting via the EP4 receptor

derived cells (CDCs) are a cardiac progenitor cell population, which have been shown to possess cardiac regenerative properties and can improve heart function in a variety of cardiac diseases. Studies in large animal models have predominantly focussed on using autologous cells for safety, however allogeneic cell banks would allow for a practical, cost-effective and efficient use in a clinical setting. The aim of this work was to determine the immunomodulatory status of these cells using CDCs and lymphocytes from 5 dogs. CDCs expressed MHC I but not MHC II molecules and in mixed lymphocyte reactions demonstrated a lack of lymphocyte proliferation in response to MHC-mismatched CDCs. Furthermore, MHC-mismatched CDCs suppressed lymphocyte proliferation and activation in response to Concanavalin A. Transwell experiments demonstrated that this was predominantly due to direct cell-cell contact in addition to soluble mediators whereby CDCs produced high levels of PGE2 under inflammatory conditions. This led to down-regulation of CD25 expression on lymphocytes via the EP4 receptor. Blocking prostaglandin synthesis restored both, proliferation and activation (measured via CD25 expression) of stimulated lymphocytes. We demonstrated for the first time in a large animal model that CDCs inhibit proliferation in allo-reactive lymphocytes and have potent immunosuppressive activity mediated via PGE2

Two Energy Scales and two Quasiparticle Dynamics in the Superconducting State of Underdoped Cuprates

The superconducting state of underdoped cuprates is often described in terms of a single energy-scale, associated with the maximum of the (d-wave) gap. Here, we report on electronic Raman scattering results, which show that the gap function in the underdoped regime is characterized by two energy scales, depending on doping in opposite manners. Their ratios to the maximum critical temperature are found to be universal in cuprates. Our experimental results also reveal two different quasiparticle dynamics in the underdoped superconducting state, associated with two regions of momentum space: nodal regions near the zeros of the superconducting gap and antinodal regions. While antinodal quasiparticles quickly loose coherence as doping is reduced, coherent nodal quasiparticles persist down to low doping levels. A theoretical analysis using a new sum-rule allows us to relate the low-frequency-dependence of the Raman response to the temperature-dependence of the superfluid density, both controlled by nodal excitations.Comment: 16 pages, 5 figure

Conformational Space and Stability of ETD Charge Reduction Products of Ubiquitin

Owing to its versatility, electron transfer dissociation (ETD) has become one of the most commonly utilized fragmentation techniques in both native and non-native top-down mass spectrometry. However, several competing reactions—primarily different forms of charge reduction—occur under ETD conditions, as evidenced by the distorted isotope patterns usually observed. In this work, we analyze these isotope patterns to compare the stability of nondissociative electron transfer (ETnoD) products, specifically noncovalent c/z fragment complexes, across a range of ubiquitin conformational states. Using ion mobility, we find that more extended states are more prone to fragment release. We obtain evidence that for a given charge state, populations of ubiquitin ions formed either directly by electrospray ionization or through collapse of more extended states upon charge reduction, span a similar range of collision cross-sections. Products of gas-phase collapse are, however, less stabilized towards unfolding than the native conformation, indicating that the ions retain a memory of previous conformational states. Furthermore, this collapse of charge-reduced ions is promoted if the ions are ‘preheated’ using collisional activation, with possible implications for the kinetics of gas-phase compaction

The GAA triplet-repeat is unstable in the context of the human FXN locus and displays age-dependent expansions in cerebellum and DRG in a transgenic mouse model

Friedreich ataxia (FRDA) is caused by homozygosity for FXN alleles containing an expanded GAA triplet-repeat (GAA-TR) sequence. This expanded GAA-TR sequence is unstable in somatic cells of FRDA patients, showing age-dependent expansions in dorsal root ganglia (DRG), the tissue where pathology occurs earliest and is most significant. This is thought to be the basis for the progressive, tissue-specific pathology seen in FRDA, but the mechanism(s) for this somatic instability is unknown. We show that transgenic mice containing the expanded GAA-TR sequence (190 or 82 triplets) in the context of the human FXN locus show tissue-specific and age-dependent somatic instability that mimics the human condition. Small pool PCR analysis, which allows quantitative analysis of instability by assaying individual transgenes in vivo, showed age-dependent expansions specifically in the cerebellum and DRG. The (GAA)190 allele showed some instability by 2 months, progressed at about 0.3 – 0.4 triplets/week, resulting in a significant number of expansions by 12 months. Repeat length determined the age of onset of somatic instability, and the rate and magnitude of expansion. Whereas the GAA-TR was unstable in the context of the human FXN locus, pure GAATR sequences at other genetic loci in the human and murine genomes showed no instability. These data indicate that somatic instability of the GAA-TR sequence in the human FXN gene is determined by a combination of unique cis and trans-acting factors. This mouse model will serve as a useful tool to delineate the mechanism(s) of diseasespecific somatic instability in FRDA

Friedreich's Ataxia (GAA)n•(TTC)n Repeats Strongly Stimulate Mitotic Crossovers in Saccharomyces cerevisae

Expansions of trinucleotide GAA•TTC tracts are associated with the human disease Friedreich's ataxia, and long GAA•TTC tracts elevate genome instability in yeast. We show that tracts of (GAA)230•(TTC)230 stimulate mitotic crossovers in yeast about 10,000-fold relative to a “normal” DNA sequence; (GAA)n•(TTC)n tracts, however, do not significantly elevate meiotic recombination. Most of the mitotic crossovers are associated with a region of non-reciprocal transfer of information (gene conversion). The major class of recombination events stimulated by (GAA)n•(TTC)n tracts is a tract-associated double-strand break (DSB) that occurs in unreplicated chromosomes, likely in G1 of the cell cycle. These findings indicate that (GAA)n•(TTC)n tracts can be a potent source of loss of heterozygosity in yeast

Identification of a Novel Aminopeptidase P-Like Gene (OnAPP) Possibly Involved in Bt Toxicity and Resistance in a Major Corn Pest (Ostrinia nubilalis)

Studies to understand the Bacillus thuringiensis (Bt) resistance mechanism in European corn borer (ECB, Ostrinia nubilalis) suggest that resistance may be due to changes in the midgut-specific Bt toxin receptor. In this study, we identified 10 aminopeptidase-like genes, which have previously been identified as putative Bt toxin receptors in other insects and examined their expression in relation to Cry1Ab toxicity and resistance. Expression analysis for the 10 aminopeptidase-like genes revealed that most of these genes were expressed predominantly in the larval midgut, but there was no difference in the expression of these genes in Cry1Ab resistant and susceptible strains. This suggested that altered expression of these genes was unlikely to be responsible for resistance in these ECB strains. However, we found that there were changes in two amino acid residues of the aminopeptidase-P like gene (OnAPP) involving Glu305 to Lys305 and Arg307 to Leu307 in the two Cry1Ab-resistant strains as compared with three Cry1Ab-susceptible strains. The mature OnAPP contains 682 amino acid residues and has a putative signal peptide at the N-terminus, a predicted glycosylphosphatidyl-inositol (GPI)-anchor signal at the C-terminal, three predicted N-glycosylation sites at residues N178, N278 and N417, and an O-glycosylation site at residue T653. We used a feeding based-RNA interference assay to examine the role of the OnAPP gene in Cry1Ab toxicity and resistance. Bioassays of Cry1Ab in larvae fed diet containing OnAPP dsRNA resulted in a 38% reduction in the transcript level of OnAPP and a 25% reduction in the susceptibility to Cry1Ab as compared with larvae fed GFP dsRNA or water. These results strongly suggest that the OnAPP gene could be involved in binding the Cry1Ab toxin in the ECB larval midgut and that mutations in this gene may be associated with Bt resistance in these two ECB strains

The MYST-Containing Protein Chameau Is Required for Proper Sensory Organ Specification during Drosophila Thorax Morphogenesis

The adult thorax of Drosophila melanogaster is covered by a stereotyped pattern of mechanosensory bristles called macrochaetes. Here, we report that the MYST containing protein Chameau (Chm) contributes to the establishment of this pattern in the most dorsal part of the thorax. Chm mutant pupae present extra-dorsocentral (DC) and scutellar (SC) macrochaetes, but a normal number of the other macrochaetes. We provide evidences that chm restricts the singling out of sensory organ precursors from proneural clusters and genetically interacts with transcriptional regulators involved in the regulation of achaete and scute in the DC and SC proneural cluster. This function of chm likely relies on chromatin structure regulation since a protein with a mutation in the conserved catalytic site fails to rescue the formation of supernumerary DC and SC bristles in chm mutant flies. This is further supported by the finding that mutations in genes encoding chromatin modifiers and remodeling factors, including Polycomb group (PcG) and Trithorax group (TrxG) members, dominantly modulate the penetrance of chm extra bristle phenotype. These data support a critical role for chromatin structure modulation in the establishment of the stereotyped sensory bristle pattern in the fly thorax